During the second half of 2013 a total of 26 deaths including isomer that also appeared to be involved in at least 18 of the 26 deaths. concerning the detection and characterization of 4-MAR on the other hand began to appear following a case statement about a fatality in the late 1980s. The presence of two chiral carbons in 4-MAR gives rise to two diastereomeric racemates that have been previously studied.[19-23] The preparation and characterization of the four 3 4 enantiomers i.e. no substituent within the phenyl ring have also Razaxaban been explained. racemates in order to establish the potential for unambiguous differentiation less than routine analytical conditions (Number 1C). A very recent test purchase from an alternative online merchant also confirmed that (±)-= 8.0 Hz 2 H Ar H) 7.16 (d = 8.0 Hz 2 H Ar H) 4.53 (d = 4.3 Hz 1 H CH(OH)) 3.16 (m 1 H CH(CH3)) 2.35 (s 3 H Ar-CH3) and 0.99 (d = 6.5 Hz 3 H CH(CH3)); 13C NMR (CDCl3) δ 138.19 (Ar C) 136.09 (Ar C) 128.71 (Ar CH) 126.3 (Ar CH) 77.17 (CH(OH)) 51.86 (CH(CH3)) 20.94 (Ar-CH3) and 17.84 (CH(CH3)); HR-ESIMS found 166.1222 (theor. for [M+H]+ C11H16NO 166.1226 (±)-= 7.8 Hz 2 H Ar H) 7.12 (d = 7.8 Hz 2 H Ar H) 5.74 (d = 8.7 Mdk Hz H-5) 4.41 (dq = 8.7 6.8 Hz H-4) 2.38 (s 3 H Ar-CH3) and 0.84 (d = 6.8 Hz 3 H CH3); 13C NMR (CDCl3) δ 160.90 (C-2) 138.3 (Ar C) 131.71 (Ar C) 129.04 (Ar CH) 125.85 (Ar CH) 85.59 (C-5) 59.5 (C-4) 21.07 (Ar-CH3) and 17.59 (CH3); HR-ESIMS found 191.1175 (theor. for [M+H]+ C10H15N2O 191.1179 (±)-= 7.7 Hz 1 H H-5) 4.05 (dq J = 7.7 6.2 Hz 1 H H-4) 2.38 (s 3 H Razaxaban Ar-CH3) and 1.40 (d = 6.2 Hz 3 H CH3); 13C Razaxaban NMR (CDCl3) δ 160.49 (C-2) 139.34 (Ar C) 133.84 (Ar C) 129.76 (Ar CH) 126.31 (Ar CH) 90.25 (C-5) 63.71 (C-4) 21.03 (Ar-CH3) and 20.08 (CH3); HR-ESIMS found 191.1176 (theor. for [M+H+] C10H15N2O 191.1179 Instrumentation Gas chromatography ion capture mass spectrometry GC ion capture MS data for (±)-41-500) using a Varian 450-GC gas chromatograph coupled to a Varian 220-MS ion capture mass spectrometer. A Varian 8400 autosampler was used having a CP-1177 injector (275 Razaxaban °C) in break up mode (1:50). Data acquisition was performed with the MS Data Review function of the Workstation software version 6.91. Transfer collection manifold and ion capture temps were arranged at 310 80 and 220 °C respectively. The liquid CI reagent was HPLC grade methanol. CI ionization guidelines (0.4 s/check out): CI storage level 19.0 191 and four product ions were collected i.e. at m/z Razaxaban 148 m/z 131 m/z 91and m/z 56 respectively Dwell time for each channel was 0.05 s. Interchannel delay was 0.02 s. The 4 4 compounds were also injected by direct infusion (10 μL/min) in order to obtain the related product ions. The standard LC-MS/MS settings were applied however people were collected over the range between 45 and 200; collision voltage 23 eV; desolvation temp 200°C; desolvation gas circulation 200 L/h. Liquid chromatography electrospray solitary quadrupole mass spectrometry LC-MS analyses were performed on an Agilent 1100 LC system. Separation was acquired on an Allure PFP Propyl column (5 μm 50 mm × 2.1 mm) from Restek (Bellefonte PA USA) and the aqueous mobile phase A consisted of 0.05% formic acid in water whereas mobile phase B was prepared from 0.05% formic acid in acetonitrile respectively. The Agilent LC-MSD settings were as follows: positive electrospray mode capillary voltage 3000 V drying gas (N2) 12 L/min at 350 °C nebulizer gas (N2) pressure 60 psi EIC 191 and 148 fragmentor voltage 70 V. Samples for LC-MS analysis (1 μL injection volume) were dissolved in acetonitrile/water (1:1 comprising 0.1% formic acid) at a concentration of 5 μg/ml. The following gradient elution system was used: 0-4 min 2% A then increase to 30% over 30 min using a linear gradient. The circulation rate was 1 mL/min and the column temp was 30 °C. High-resolution electrospray mass spectrometry HR-ESI mass spectra for the synthesized (±)-100-1000 with and without auto MS/MS fragmentation. Ionization was accomplished with an Agilent JetStream electrospray resource and infused internal reference people. Agilent 6540 Q-TOF-MS guidelines: gas temp 325 °C drying gas 10 L/min and sheath gas temp 400 °C. Internal research masses of.