Supplementary MaterialsFigure 1source data 1: An Excel sheet with numerical data over the quantification of the result of centrinone treatment over the EC mean intensity of -tubulin sign, MT dynamics parameters, EB comet number, the polarization of Golgi during migration, the efficiency of wound closure, the cumulative amount of spheroid sprouts as well as the proportion of sprouting ECs with centrosome along with the aftereffect of CPAP depletion over the cumulative amount of spheroid sprouts represented as plots in Amount 1ACF,H. Amount 1figure dietary supplement 1ACompact disc. elife-33864-fig1-figsupp1-data1.xlsx (16K) DOI:?10.7554/eLife.33864.007 Figure 1figure supplement 2source data 1: An Excel sheet with numerical data over the quantification of the result of centrinone treatment over the expression of CAMSAP2 and different post-translationally modified tubulin in ECs, the mean strength of acetylated tubulin signal, the thickness of CAMSAP2 stretches, the strength of VE-Cadherin and 1M7 ZO-1 signal at cell junctions, the directionality and velocity of cell migration during scratch-wound assays, along with the aftereffect of CPAP depletion on centrosome removal efficiency as well as the percentage of 3D sprouting ECs with centrosome represented as plots in Figure 1figure supplement 2ACG. elife-33864-fig1-figsupp2-data1.xlsx (19K) DOI:?10.7554/eLife.33864.008 Figure 2source data 1: An Excel sheet with numerical data over the quantification of the result of CAMSAP2 silencing on MT dynamics variables, the efficiency of wound closure, the cumulative amount of spheroid sprouts, their number and average length, as well as the cumulative amount of spheroid sprouts re-expressing CAMSAP2 represented as plots in Figure 2D,E,G,H. elife-33864-fig2-data1.xlsx (18K) DOI:?10.7554/eLife.33864.012 Figure 2figure dietary supplement 1source data 1: An Excel sheet with numerical data over the quantification of CAMSAP2 stretch out amount and duration after VEGF treatment, along with the aftereffect of CAMSAP2 depletion over the EC mean strength of -tubulin indication, EB comet amount, the appearance of CAMSAP2 and different modified tubulin post-translationally, MT nucleation activity and EB3 Golgi and centrosome enrichment after nocodazole washout, the true number, speed and amount of KIF13B monitors as well as the speed of cell migration during scratch-wound assays represented as plots in Figure 2figure dietary supplement 1A,C,E,F,H,I. elife-33864-fig2-figsupp1-data1.xlsx (20K) DOI:?10.7554/eLife.33864.013 Amount 2figure dietary supplement 2source data 1: An Excel sheet with numerical data over the quantification from the EC mitotic index and doubling period after CAMSAP2 depletion, the cumulative amount of spheroid sprouts after CAMSAP2 depletion and treatment with thymidine and after CAMSAP2 and CAMSAP3 depletion represented as story in Amount 2figure health supplement 2A,B,D. elife-33864-fig2-figsupp2-data1.xlsx (18K) DOI:?10.7554/eLife.33864.014 Figure 3source data 1: An Excel sheet with numerical data in the quantification of the result of CAMSAP2 depletion in the 3D elongation of ECs, the real amount of their 3D protrusions and along the longest one, their polarity index (protrusion organization), the persistence from NSHC the protrusions as time passes as well as the enrichment of -tubulin signal within the longest protrusion represented as plots in Figure 3B,C,D,F,G. elife-33864-fig3-data1.xlsx (22K) DOI:?10.7554/eLife.33864.017 Body 3figure health supplement 1source data 1: An Excel sheet with numerical data in the quantification of the result of CAMSAP2 depletion on the 1M7 quantity and amount of spheroid protrusions as time passes, the full total and typical amount of the 3D protrusions of isolated ECs, the binning of the common protrusion duration by their path, the polarity index from the 3D protrusions with regards to their duration and the amount of 3D protrusions as time passes represented as plots in Body 3figure health supplement 1A,C,ECG. elife-33864-fig3-figsupp1-data1.xlsx (26K) DOI:?10.7554/eLife.33864.018 Body 4source data 1: An Excel sheet with numerical data in the quantification of the result of CAMSAP2 depletion in the strength of phalloidin signal in 2D (mean strength) and in 3D (optimum strength) ECs, in addition to in the EC polarity index, the persistence from the protrusions as time passes as well as the cumulative amount of spheroid sprouts after Y27632 and blebbistatin treatment represented as plots in Body 4A,CCF. elife-33864-fig4-data1.xlsx (33K) DOI:?10.7554/eLife.33864.022 Body 4figure health supplement 1source data 1: An Excel sheet with numerical data in the quantification of the result of CAMSAP2 depletion in the percentage of coverage as well as the width of lamellipodia, the cumulative duration as well as the width of tension fibres in 2D migrationg 1M7 ECs, the activation degree of Rac1 and Rho GTPases, the strength of VE-Cadherin and ZO-1 sign at cell junctions as well as the strength of phalloidin sign in 3D represented as plots (or mean worth??SD for 1B) in Body 4figure health supplement 1ACompact disc. elife-33864-fig4-figsupp1-data1.xlsx (17K) DOI:?10.7554/eLife.33864.023 Body 4figure health supplement 2source data 1: An Excel sheet with numerical data in the quantification of the result of CAMSAP2 depletion and Y632 or blebbistatin treatment in the cumulative and average amount of 3D protrusions from isolated EC, their 1M7 amount and along the longest ones in addition to on the quantity and amount of spheroid sprouts symbolized as story in Body 4figure health supplement 2BCF. elife-33864-fig4-figsupp2-data1.xlsx (24K) DOI:?10.7554/eLife.33864.024 Body 5source data 1: An Excel sheet with numerical data in the quantification of the result of CAMSAP2 depletion in the directionality of EC migration, the correlation between your position from the lamellipodia, Golgi and wound during migration, the percentage of Rab6 paths in leading of migrating ECs,.
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